Application
Immunocytochemistry/Immunofluorescence: MTH1 Antibody - Detection of MTH1 (Green) in Hela cells using . Nuclei (Blue) are counterstained with Hoechst 33258.Application
Western Blot: MTH1 Antibody - MTH1 antibody was tested in the labeled lanes.Application
Western Blot: MTH1 Antibody - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate| Product Name | MTH1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MTH1Oxygen radicals damage chromosomal DNA causing cell death and inducing mutations. Among the various classes of DNA damage caused by oxygen radicals, an oxidized form of guanine base (8-oxoguanine) appears to be important as it can pair with cytosine and adenine and G:C to T:A transversion mutation occurs. A significant amount of 8-OxoG is formed in the chromosomal DNA of mammalian cells, with most damaged nucleotides excised from the DNA and excreted in the urine. Along with 8-oxoG being present in oxidatively damaged DNA, 8-oxo-deoxyguanosine (8-oxo-dGTP) is formed in the nucleotide pool during normal cellular metabolism and following oxidative stress. The 8-oxo-dGTP nucleotide can be incorporated in DNA during polymerization and can result in a mispairing unless repaired. MTH converts 8-oxo-dGTP in the nucleotide pool to the monophosphate and prevents the misincorporation of 8-oxo-dGTP into DNA (Figure courtesy of Dr. Mark Kelley). MTH also recognizes 8-oxo-rGTP, which could incorporate into RNA during gene transcription leading to missense or nonsense protein production. |
| Immunogen | A c-terminal peptide derived from the human MTH1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FWB |
| For WB: Use at a dilution of 1:500. Can be used for Western blot detection of MTH1 where a band is seen at ~ 18 kDa. For IHC-Fr: Use at a dilution of 1:200. Optimal dilutions/concentrations shuld be determined by the researcher.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MTH1 4521 P36639 NUDT1 600312 |
|---|---|
| Target | MTH1 |
| Tissue Specificity | Is specific for MTH1. |
Application
Immunocytochemistry/Immunofluorescence: MTH1 Antibody - Detection of MTH1 (Green) in Hela cells using . Nuclei (Blue) are counterstained with Hoechst 33258.
Application
Western Blot: MTH1 Antibody - MTH1 antibody was tested in the labeled lanes.
Application
Western Blot: MTH1 Antibody - Analysis of MTH1 in 1. Ntera2, 2. MCF7 cell lysate, 3. A431 cell lysate and 4. COS7 cell lysate| Positive Control | Hela cell extracts |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500. Can be used for Western blot detection of MTH1 where a band is seen at ~ 18 kDa. For IHC-Fr: Use at a dilution of 1:200. Optimal dilutions/concentrations shuld be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffereed saline containing 0.05% Sodium Azide |
