Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.Application
Untreated (–) and treated (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody diluted at 1:1000.| Product Name | mTOR (phospho Ser2448) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to mTOR (phospho Ser2448) (mechanistic target of rapamycin (serine/threonine kinase))The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human mTOR (phospho Ser2448). The exact sequence is proprietary. |
| Modification | p-Ser2448 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, SheepMouse (100%), Rat (100%), Sheep (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Target | mTOR |
|---|---|
| Molecular Weight(MW) | 289 kDa (note) |
| Cellular Localization | Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side , Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side , Mitochondrion outer membrane; Peripheral membrane protein; Cytoplasmic side |
Application
Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.
Application
Untreated (–) and treated (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with mTOR (phospho Ser2448) antibody diluted at 1:1000.| Positive Control | 293T , 293T (200 ng/ml EGF treatment for 0.5 hr) |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
