Application
at a 1:100 dilutionconfocally stainingmTOR (red)in L6 myotubes,alongside a nuclear antigen antibody(green).Application
Immunoprecipitation was performed using (1:1000 dilution) for use in Kinase Assays. The sample was recombinant 4E-BP1, incubated at 30℃ with mTOR immunoprecipitate and 32P-gamma ATP. Detection was performed using fluorography.Application
'Western Blot: mTOR Antibody - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti-mTOR antibody used for WB at 0.4 ug/ml (A) used for IP at 3 ug/mg lysate. mTOR was also immunoprecipitated by a previous lot of this antibody and by rabbit anti-mTOR antibodies. For blotting immunoprecipitated mTOR, was used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).'| Product Name | mTOR antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to mTORFKBP 12 rapamycin associated protein (FRAP) is one of a family of proteins involved in cell cycle progression, DNA recombination, and DNA damage detection. In rat, it is a 235 kDa protein (symbolized RAFT 1) with significant homology to the Saccharomyces cerevisiae protein TOR 1 and has been shown to associate with the immunophilin FKBP 12 in a rapamycin-dependent fashion (1). The FKBP 12 rapamycin complex is known to inhibit progression through the G1 cell cycle stage by interfering with mitogenic signaling pathways involved in G1 progression in several cell types, as well as in yeast (2). The binding of FRAP to FKBP 12 rapamycin correlated with the ability of these ligands to inhibit cell cycle progression (2). |
| Immunogen | Synthetic peptide (Human) - which represents a portion of human Mammalian Target of Rapamycinencoded in part by exons 5 and 6. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | RAPT1 601231 2475 P42345 MTOR FRAP FRAP2 FRAP1 RAFT1 |
|---|---|
| Target | mTOR |
Application
at a 1:100 dilutionconfocally stainingmTOR (red)in L6 myotubes,alongside a nuclear antigen antibody(green).
Application
Immunoprecipitation was performed using (1:1000 dilution) for use in Kinase Assays. The sample was recombinant 4E-BP1, incubated at 30℃ with mTOR immunoprecipitate and 32P-gamma ATP. Detection was performed using fluorography.
Application
'Western Blot: mTOR Antibody - Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Affinity purified rabbit anti-mTOR antibody used for WB at 0.4 ug/ml (A) used for IP at 3 ug/mg lysate. mTOR was also immunoprecipitated by a previous lot of this antibody and by rabbit anti-mTOR antibodies. For blotting immunoprecipitated mTOR, was used at 1 ug/ml. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 minutes (B).'| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Tris-citrate/phosphate, pH 7-8, containing 0.1% sodium azide |
