Application
Immunofluorescence analysis of methanol-fixed Hep G2, using mtRNA polymerase antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using mtRNA polymerase antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: HepG2 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | mtRNA polymerase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to mtRNA polymerase (polymerase (RNA) mitochondrial (DNA directed))This gene encodes a mitochondrial DNA-directed RNA polymerase. The gene product is responsible for mitochondrial gene expression as well as for providing RNA primers for initiation of replication of the mitochondrial genome. Although this polypeptide has the same function as the three nuclear DNA-directed RNA polymerases, it is more closely related to RNA polymerases of phage and mitochondrial polymerases of lower eukaryotes. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human mtRNA polymerase. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| WB:1:500~1:1000 IHC-p:1:50~1:200 ICC/IF:1:100~1:500 *Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | APOLMT antibody MTRNAP antibody MTRPOL antibody h-mtRPOL antibody POLRMT antibody |
|---|---|
| Molecular Weight(MW) | 139 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunofluorescence analysis of methanol-fixed Hep G2, using mtRNA polymerase antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using mtRNA polymerase antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: HepG2 5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HepG2 |
|---|---|
| Application Notes | WB:1:500~1:1000 IHC-p:1:50~1:200 ICC/IF:1:100~1:500 *Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
