Application
mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.Application
mtTFA antibody detects mtTFA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted by 1:1000.Application
mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.Application
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using mtTFA antibody at 1:250 dilution.| Product Name | mtTFA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to mtTFA (transcription factor A, mitochondrial)This gene encodes a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this gene product is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of this gene was eliminated by targeted disruption in heart and muscle cells. [provided by RefSeq] |
| Immunogen | Full length human mtTFA Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | ChimpanzeeChimpanzee (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MtTF1 antibody TCF6 antibody TCF6L1 antibody TCF6L2 antibody TCF6L3 antibody mtTFA antibody TFAM antibody Transcription factor 6-like 2 (mitochondrial transcription factor) antibody transcription factor A mitochondrial antibody mitochondrial tran |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
mtTFA antibody immunoprecipitates mtTFA protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of mtTFA protein by 2 μg of mtTFA antibody 12% SDS-PAGE The immunoprecipitated mtTFA protein was detected by mtTFA antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500. Red: Mitotracker, a mitochondria marker, diluted at 1:2000. Blue: Hoechst 33342 staining.
Application
mtTFA antibody detects mtTFA protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted by 1:1000.
Application
mtTFA antibody detects mtTFA protein at mitochondria by immunofluorescent analysis. Sample: NT2D1 cells were fixed in 2% paraformaldehyde/culture medium at RT for 30 min. Green: mtTFA protein stained by mtTFA antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.
Application
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with mtTFA antibody diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using mtTFA antibody at 1:250 dilution.| Positive Control | 293T , A431 , HeLa , HepG2 , Neuro 2A |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
