Application
- Extracts of rat brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with the µ-opioid receptor antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate.| Product Name | Mu Opioid receptor antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Mu Opioid ReceptorMost actions of exogenous opioids, such as morphine, are mediated through the μ-opioid receptor, including analgesia, tolerance and reward. In general, opioids modulate numerous central and peripheral processes including pain perception, neuroendocrine secretion and the immune response. The opioid signal is transduced from receptors through G proteins to various different effectors. Subsequent to G protein activation, several effectors are known to orchestrate the opioid signal. For example, activation of opioid receptors increases phosphatidylinositol turnover, activates K+ channels and reduces adenylyl cyclase and Ca++ channel activities. |
| Immunogen | Synthetic peptide (Human) (Internal sequence, ARIRQNTREHPSTAC). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1/1000. Predicted molecular weight: ~45 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MOR1 4988 P35372 OPRM 600018 Mu Opioid receptor KIAA0403 OPRM1 |
|---|---|
| Target | Mu Opioid receptor |
| Cellular Localization | Integral membrane protein. |
Application
- Extracts of rat brain lysates were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature and incubated with the µ-opioid receptor antibody for two hours at room temperature in a 1% BSA-TBST buffer. After washing, the membrane was incubated with goat F(ab’)2 anti-rabbit IgG HRP conjugate.| Positive Control | brain, spinal cord (lysate or tissue) |
|---|---|
| Application Notes | WB: 1/1000. Predicted molecular weight: ~45 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide. Constituents: 50% Glycerol, PBS, 1mg/ml BSA. pH 7.3 |
