Application
Western blot analysis of MUC1 (pY1229) expression in MCF7 PMA-treated (A); BT20 (B); NIH3T3 UV-treated (C); H9C2 PMA-treated (D) whole cell lysates.| Product Name | MUC1 (phospho Tyr1229) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MUC1 (phospho Tyr1229)This gene encodes a membrane-bound protein that is a member of the mucin family. Mucins are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. These proteins also play a role in intracellular signaling. This protein is expressed on the apical surface of epithelial cells that line the mucosal surfaces of many different tissues including lung, breast stomach and pancreas. This protein is proteolytically cleaved into alpha and beta subunits that form a heterodimeric complex. The N-terminal alpha subunit functions in cell-adhesion and the C-terminal beta subunit is involved in cell signaling. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas. This gene is known to contain a highly polymorphic variable number tandem repeats (VNTR) domain. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2011] |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MUC1. The exact sequence is proprietary. |
| Modification | p-Tyr1229 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MUC-1/SEC MAM6 MCKD1 CA 15-3 EMA PEMT mucin 1 cell surface associated ADMCKD H23AG MUC1 MCD PUM PEM CD227 Antib |
|---|---|
| Target | MUC1 (phospho Tyr1229) |
| Tissue Specificity | Recognizes endogenous levels of MUC1 (pY1229) protein. |
Application
Western blot analysis of MUC1 (pY1229) expression in MCF7 PMA-treated (A); BT20 (B); NIH3T3 UV-treated (C); H9C2 PMA-treated (D) whole cell lysates.| Application Notes | WB: 1:500 - 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4 °C. Upon delivery aliquot and store at -20 °C for one year. Avoid freeze/thaw cycles. |
| Storage Buffer | Liquid in 0.42% Potassium phosphate; 0.87% Sodium chloride; pH 7.3; 30% glycerol; and 0.01% sodium azide. |
