WB
Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate, All lanes: PEMT antibody at 2.8µg/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.ICC/IF
Immunofluorescent analysis of A549 cells using PEMT Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | PEMT Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Phosphatidylethanolamine N-methyltransferase protein (2-49AA) |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:1000-1:5000 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | PEMT |
|---|---|
| Gene Synonyms | PLMT PEAMT PEMPT PEMT2 |
| Gene Full Name | phosphatidylethanolamine N-methyltransferase |
| Gene Summary | Phosphatidylcholine (PC) is the most abundant mammalian phospholipid. This gene encodes an enzyme which converts phosphatidylethanolamine to phosphatidylcholine by sequential methylation in the liver. Another distinct synthetic pathway in nucleated cells converts intracellular choline to phosphatidylcholine by a three-step process. The protein isoforms encoded by this gene localize to the endoplasmic reticulum and mitochondria-associated membranes. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2012] |
| Molecular Weight(MW) | 23/26/25kDa(Observed MW 23kDa) |
| Cellular Localization | Endoplasmic reticulum membrane |
WB
Western Blot, Positive WB detected in: SH-SY5Y whole cell lysate, All lanes: PEMT antibody at 2.8µg/ml, Secondary, Goat polyclonal to rabbit IgG at 1/50000 dilution.
ICC/IF
Immunofluorescent analysis of A549 cells using PEMT Antibody at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | WB:1:1000-1:5000 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, pH 7.4 |
