Application
Western blot analysis of anti-PEMT Antibody (N-term) Pab in mouse kidney tissue lysates (35ug/lane). PEMT(arrow) was detected using the purified Pab (1:60 dilution).Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Product Name | PEMT Antibody (N-term) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PEMT; PEMPT; PNMT; Phosphatidylethanolamine N-methyltransferase; PEMT2 |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WBIHC |
| WB:1:50~100 IHC |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | PEMPT PNMT |
|---|---|
| Alternative Names | PEMT PEMPT PNMT Phosphatidylethanolamine N-methyltransferase PEMT2 |
| Molecular Weight(MW) | 22134 Da |
| Function | Catalyzes three sequential methylation reactions of phosphatidylethanolamine (PE) by AdoMet, thereby producing phosphatidylcholine (PC) |
| Tissue Specificity | This PEMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PEMT. |
| Cellular Localization | Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential). Mitochondrion membrane; Multi-pass membrane protein (Potential). Note=Found in endoplasmic reticulum where most PEMT activity is generated and in mitochondria (By similarity) |
| Entrez Gene | 10400 |
|---|
Application
Western blot analysis of anti-PEMT Antibody (N-term) Pab in mouse kidney tissue lysates (35ug/lane). PEMT(arrow) was detected using the purified Pab (1:60 dilution).
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with PEMT antibody (N-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.| Application Notes | WB:1:50~100 IHC |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS. |
