ICC/IF
Immunofluorescence analysis of A549 cells labelling PNMT with PNMT Rabbit mAb. The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with PNMT Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Product Name | PNMT Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide of Human PNMT. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IF |
| ICC/IF:1:100-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PNMT |
|---|---|
| Gene Synonyms | PENT PNMTase |
| Gene Full Name | phenylethanolamine N-methyltransferase |
| Gene Summary | The product of this gene catalyzes the last step of the catecholamine biosynthesis pathway, which methylates norepinephrine to form epinephrine (adrenaline). The enzyme also has beta-carboline 2N-methyltransferase activity. This gene is thought to play a key step in regulating epinephrine production. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012] |
| Alternative Names | PENT PNMTase |
| Molecular Weight(MW) | 31 kDa |
ICC/IF
Immunofluorescence analysis of A549 cells labelling PNMT with PNMT Rabbit mAb. The cells were fixed with cold 100% methanol (10min, 4℃) and blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween 20 for 1h. The cells were then incubate with PNMT Rabbit mAb (1:100) at room temprature for 1h, followed by a further incubation at room temperature for 45min with Goat Anti Rabbit lgG (H+L)-AF488 (shown in green). Nuclear DNA was labeled in blue with DAPI. Control: Secondary antibody only.| Application Notes | ICC/IF:1:100-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C.Stable for 12 months from date of receipt. |
| Storage Buffer | Supplied in 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
