Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUL1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.Application
MUL1 antibody detects MUL1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. MUL1 antibody dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MUL1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MUL1 (mitochondrial E3 ubiquitin protein ligase 1)E3 ubiquitin-protein ligase that plays a role in the control of mitochondrial morphology. Promotes mitochondrial fragmentation and influences mitochondrial localization. Inhibits cell growth. When overexpressed, activates JNK through MAP3K7/TAK1 and induces caspase-dependent apoptosis. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer the ubiquitin to targeted substrates. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MUL1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (90%), Rat (91%), Rhesus Monkey (100%), Bovine (82%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C1orf166 antibody FLJ12875 antibody GIDE antibody MAPL antibody MULAN antibody RNF218 antibody RP11-401M16.2 antibody MUL1 antibody growth inhibition and death E3 ligase antibody E3 ubiquitin ligase antibody E3 SUMO-protein ligase MUL1 antibody |
|---|---|
| Molecular Weight(MW) | 40 kDa (note) |
| Cellular Localization | Mitochondrion outer membrane; Multi-pass membrane protein , Peroxisome |
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using MUL1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:500.
Application
MUL1 antibody detects MUL1 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. MUL1 antibody dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: A431 10% SDS PAGE diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
