Application
Western blot of Munc-18 in rat brain homogenate immunoprecipitated with 1. Extract only. 2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of respectively.| Product Name | Munc18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Munc18Munc-18 is a cytosolic brain protein of ~67 kDa which plays a key role in vesicle membrane fusion events with the plasma membrane. Functional interactions have been demonstrated to exist between Munc-18, synaptosomal associated protein of 25 kDa (SNAP-25), synaptobrevin, and syntaxin. Munc-18 is the mammalian homologue of the C. elegans gene unc-18. Mutations in Munc-18 can result in accumulations of acetylcholine and paralytic phenotype suggestive of a neurotransmitter release defect. Munc-18 thus appears to be required by the synaptic vesicle fusion complex. Two Munc-18 isoforms have recently been identified. Munc-18-1 has been shown to be primarily expressed in brain, while Munc-18-2 is expressed in most tissues. |
| Immunogen | Synthetic Peptide: V(58) E D I N K R R E P I P S(70) |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIHC-FIPWB |
| WB: 1:7500 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | HumanMouseDogRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | nsec1 Stxbp1 Sec1 n-sec1 Munc18 rbSec1 P61764 MUNC18-1 NSEC1A Munc181 UNC18 602926 ANC18HA rbSec1A hUNC18 6812 25558 rbSec1B n sec1 |
|---|---|
| Target | Munc18 |
Application
Western blot of Munc-18 in rat brain homogenate immunoprecipitated with 1. Extract only. 2-4. 750 ug of extract immunoprecipitated with 1 ug, 5 ug, and 10 ug of respectively.| Application Notes | WB: 1:7500 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 1 mg/ml BSA and 0.05% sodium azide. |
