Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE diluted at 1:3000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using STXBP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | Munc18-1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Munc18-1 (syntaxin binding protein 1)This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Munc18-1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Chicken, Rhesus Monkey, BovineRat (100%), Xenopus laevis (93%), Chicken (98%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ37475 antibody MUNC18-1 antibody NSEC1 antibody P67 antibody RBSEC1 antibody UNC18 antibody STXBP1 antibody protein unc-18 homolog 1 antibody N-Sec1 antibody unc18-1 antibody unc-18A antibody protein unc-18 homolog A antibody neuronal SEC1 a |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Membrane; Peripheral membrane protein |
Application
Sample (20 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE diluted at 1:10000
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE diluted at 1:3000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using STXBP1 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | 293T , A431 , Jurkat , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
