Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MUS81 antibody detects MUS81 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE MUS81 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MUS81 antibody detects MUS81 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MUS81 protein stained by MUS81 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.| Product Name | MUS81 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MUS81 (MUS81 endonuclease homolog (S. cerevisiae)) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MUS81. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | ChimpanzeeChimpanzee (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ21012 antibody FLJ44872 antibody SLX3 antibody MUS81 antibody SLX3 structure-specific endonuclease subunit homolog antibody crossover junction endonuclease MUS81 antibody MUS81 endonuclease homolog (S. cerevisiae) antibody |
|---|---|
| Molecular Weight(MW) | 61 kDa (note) |
| Cellular Localization | Nucleus , nucleolus |
Application
Sample (30 µg of whole cell lysate) A: HepG2 7.5% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MUS81 antibody detects MUS81 protein by western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 7.5% SDS-PAGE MUS81 antibody dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MUS81 antibody detects MUS81 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MUS81 protein stained by MUS81 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.| Positive Control | HepG2 , NIH-3T3 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
