Application
Musashi 1 antibody [N3C3] detects Musashi 1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Musashi 1 protein stained by Musashi 1 antibody [N3C3] diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .Application
MSI1 antibody [N3C3] detects MSI1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSI1 protein stained by MSI1 antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Application
MSI1 antibody [N3C3] detects MSI1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. MSI1 antibody [N3C3] diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using MSI1 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Musashi 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Musashi 1 (musashi homolog 1 (Drosophila))This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Musashi 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, BovineRat (100%), Xenopus laevis (81%), Pig (98%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MSI1 antibody musashi-1 antibody RNA-binding protein Musashi homolog 1 antibody musashi1 antibody musashi homolog 1 (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) |
Application
Musashi 1 antibody [N3C3] detects Musashi 1 protein by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: Musashi 1 protein stained by Musashi 1 antibody [N3C3] diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI .
Application
MSI1 antibody [N3C3] detects MSI1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSI1 protein stained by MSI1 antibody [N3C3] diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.
Application
MSI1 antibody [N3C3] detects MSI1 protein on adult mouse brain by immunohistochemical analysis. Sample: Frozen section of adult mouse brain. MSI1 antibody [N3C3] diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A549, using MSI1 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: H1299 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , H1299 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
