Application
Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE diluted at 1:1000| Product Name | Muscleblind-like 1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Muscleblind-like 1 (muscleblind-like (Drosophila))Mediates pre-mRNA alternative splicing regulation. Acts either as activator or repressor of splicing on specific pre-mRNA targets. Inhibits cardiac troponin-T (TNNT2) pre-mRNA exon inclusion but induces insulin receptor (IR) pre-mRNA exon inclusion in muscle. Antagonizes the alternative splicing activity pattern of CELF proteins. Regulates the TNNT2 exon 5 skipping through competition with U2AF2. Inhibits the formation of the spliceosome A complex on intron 4 of TNNT2 pre-mRNA. Binds to the stem-loop structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Binds to the 5'-YGCU(U/G)Y-3'consensus sequence. Binds to the IR RNA. Binds to expanded CUG repeat RNA, which folds into a hairpin structure containing GC base pairs and bulged, unpaired U residues. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Muscleblind-like 1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Chicken, BovineMouse (98%), Rat (99%), Zebrafish (85%), Chicken (97%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686P06174 antibody EXP antibody EXP35 antibody EXP40 antibody EXP42 antibody KIAA0428 antibody MBNL antibody MBNL1 antibody triplet-expansion RNA-binding protein antibody muscleblind-like protein 1 antibody muscleblind-like (Drosophila) anti |
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| Target | Muscleblind-like 1 |
| Molecular Weight(MW) | 42 kDa (note) (6) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: JurKat 10% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , Jurkat |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
