Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MUTYH antibody [N3C3] detects MUTYH protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MUTYH protein stained by MUTYH antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | MUTYH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MYH (mutY homolog (E. coli))This gene encodes a DNA glycosylase involved in oxidative DNA damage repair. The enzyme excises adenine bases from the DNA backbone at sites where adenine is inappropriately paired with guanine, cytosine, or 8-oxo-7,8-dihydroguanine, a major oxidatively damaged DNA lesion. The protein is localized to the nucleus and mitochondria. Mutations in this gene result in heritable predisposition to colon and stomach cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MUTYH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CYP2C antibody MGC4416 antibody MYH antibody MUTYH antibody A/G-specific adenine DNA glycosylase antibody mutY homolog (E. coli) antibody |
|---|---|
| Target | MUTYH |
| Molecular Weight(MW) | 60 kDa (note) (11) |
| Cellular Localization | Nucleus |
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MUTYH antibody [N3C3] detects MUTYH protein at mitochondria and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MUTYH protein stained by MUTYH antibody [N3C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
