| Product Name | MVP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [0.N.389] to MVPMajor vault protein (MVP) is identical to lung resistance related protein (LRP). Its shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocortoid receptors are also able to co-immunoprecipitate the MVP. Reportedly, the lung resistance related protein (LRP) is identical to human MVP. LRP is overexpressed in many neoplastic tissues and cells lines indicating a poor response to chemotherapy. |
| Immunogen | Proteins purified from extract of human breast cancer MCF-7 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 0.N.389 |
| Host Species | Mouse |
| Tested Applications | IHC-PIPWB |
| IHC-P: Use at a concentration of 2-4 μg/ml. IP: Use at a concentration of 2 μg/ml. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 104-110 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Synonyms | Q14764 LRP 605088 VAULT1 MVP 9961 |
|---|---|
| Target | MVP |
| Cellular Localization | Cytoplasmic, 5% are nucleus associated and localize to the nuclear pore complexes |
| Light Chain Type | kappa |
| Application Notes | IHC-P: Use at a concentration of 2-4 μg/ml. IP: Use at a concentration of 2 μg/ml. WB: Use at a concentration of 1 μg/ml. Detects a band of approximately 104-110 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.2% BSA, 10mM PBS, 40% glycerol, pH 7.4 |
