Application
Immunofluorescence showing major vault protein in HeLa cells using Mouse anti Human MVP :Azide FreeApplication
Staining of paraffin embedded human lung adenosquamous cell carcinoma with Mouse anti MVP :Azide FreeApplication
Western blot analysis of major vault protein expression in HeLa using Mouse anti Human MVP :Azide Free| Product Name | MVP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1014] to MVPMajor vault protein (MVP)is identical to lung-resistance related protein (LRP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70% of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Anti-estrogen1C1182 shows no effect. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. Reportedly the Lung-Resistance Related Protein (LRP)is identical to human MVP. LRP is a protein overexpressed in many neoplastic tissues and cell lines. Expression of LRP predicts a poor response to chemotherapy. |
| Immunogen | Proteins precipitated with AER 317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1014 |
| Host Species | Mouse |
| Tested Applications | FACSGSAICC/IFIHC-PIPWB |
| Flow Cytometry: assay dependent. Immunoprecipitation: use at a concentration of 2μg / mg. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 104 - 110 kDa, which corresponds to the predicted molecular weight of LRP / MVP.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Protein G |
| Gene Synonyms | Q14764 LRP 605088 VAULT1 MVP 9961 |
|---|---|
| Target | MVP |
| Cellular Localization | Cytoplasmic, 5% are nucleus associated and localize to the nuclear pore complexes |
| Light Chain Type | kappa |
Application
Immunofluorescence showing major vault protein in HeLa cells using Mouse anti Human MVP :Azide Free
Application
Staining of paraffin embedded human lung adenosquamous cell carcinoma with Mouse anti MVP :Azide Free
Application
Western blot analysis of major vault protein expression in HeLa using Mouse anti Human MVP :Azide Free| Positive Control | SKBR-3 cells |
|---|---|
| Application Notes | Flow Cytometry: assay dependent. Immunoprecipitation: use at a concentration of 2μg / mg. Western Blotting: use at a concentration of 1 - 2μg / ml for 2hrs at RT. Optimal dilutions / concentrations should be determined by the end user. By Western blot, this antibody detects a band of 104 - 110 kDa, which corresponds to the predicted molecular weight of LRP / MVP.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 10mM PBS, pH7.4, 0.2%BSA, 0.09% sodium azide |
