Application
Formalin fixed paraffin embedded human breast carcinoma stained with LRP/MVP antibody .Application
IHC Image using - Detection of MVP by IHC in Human Breast Carcinoma| Product Name | MVP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [1032] to MVPMVP is identical to Lung-Resistance Related Protein (LRP). Treatment of cells with estradiol increases the amount of MVP in nuclear extract. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. |
| Immunogen | Proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-PIPWB |
| For IHC-P: Use at a dilution of 1:50-1:100 in an ABC method for 30 mins at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Gene Synonyms | Q14764 LRP 605088 VAULT1 MVP 9961 |
|---|---|
| Target | MVP |
| Tissue Specificity | Reacts with a 104 to 110 kD protein known as Major Vault Protein (MVP). |
| Cellular Localization | Cytoplasmic |
Application
Formalin fixed paraffin embedded human breast carcinoma stained with LRP/MVP antibody .
Application
IHC Image using - Detection of MVP by IHC in Human Breast Carcinoma| Positive Control | Breast tissue sections |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:50-1:100 in an ABC method for 30 mins at RT. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. For IP: Use at an assay dependent dilution. For WB: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline containing 0.1% sodium azide |
