| Product Name | MVP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [LRP - 56] to MVPMajor vault protein (MVP) is identical to lung resistance related protein (LRP). Its shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocortoid receptors are also able to co-immunoprecipitate the MVP. Reportedly, the lung resistance related protein (LRP) is identical to human MVP. LRP is overexpressed in many neoplastic tissues and cells lines indicating a poor response to chemotherapy. |
| Immunogen | 2R120 cell line derived by stepwise Doxorubicin exposure of the the SW-1573 non-small cell lung carcinoma cell line. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | LRP-56 |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHC-F |
| FACS: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IHC-Fr: Frozen tissues, cell lines, formalin-fixed/paraffin embedded tissues. Use at a dilution of 1/10 - 1/40. Pretreatment is not required. For optimal staining, the primary antibody should be incubated overnight at 4°C. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | Q14764 LRP 605088 VAULT1 MVP 9961 |
|---|---|
| Target | MVP |
| Cellular Localization | Cytoplasmic, 5% are nucleus associated and localize to the nuclear pore complexes |
| Positive Control | Lung, Colon, kidney, bronchus or adrenal cortex (human tissue). Also in MDR cell lines such as GLC4/ADR, HT1080/DR4 & 2R120.Negative control antibody: Normal mouse serum |
|---|---|
| Application Notes | FACS: Use at an assay dependent dilution. ICC: Use at an assay dependent dilution. IHC-Fr: Frozen tissues, cell lines, formalin-fixed/paraffin embedded tissues. Use at a dilution of 1/10 - 1/40. Pretreatment is not required. For optimal staining, the primary antibody should be incubated overnight at 4°C. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | PBS with 1% BSA and 0.1% sodium azide |
