Application
Formalin fixed paraffin embedded human brain stained with MBP antibody .Application
at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed descriptApplication
at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for dApplication
Detection of Myelin basic protein by IHC in Human Breast| Product Name | Myelin basic protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Myelin Basic Protein - Oligodendrocyte MarkerThe classic group of Myelin basic protein (MBP) isoforms (isoforms 4-14) are with PLP the most abundant protein components of the myelin membrane in the CNS. They have a role in both its formation and stabilization. The smaller isoforms might have an important role in remyelination of denuded axons in multiple sclerosis. The non-classic group of MBP isoforms (isoforms 1-3/Golli-MBPs) may preferentially have a role in the early developing brain long before myelination, maybe as components of transcriptional complexes, and may also be involved in signaling pathways in T-cells and neural cells. Differential splicing events combined to optional posttranslational modifications give a wide spectrum of isomers, each of them having maybe a specialized function. |
| Immunogen | Human myelin basic protein isolated from normal human brain. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCIHC-P |
| For IHC-P: Use at a dilution of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at RT. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 4155 Myelin basic protein MBP MGC99675 P02686 159430 |
|---|---|
| Target | Myelin basic protein |
| Tissue Specificity | This antibody reacts with human myelin basic protein and oligodendrocytes of white matter in brain and spinal cord. Grey matter stains weakly. This antibody also stains peripheral nerves. |
| Cellular Localization | Cytoplasmic side of myelin |
Application
Formalin fixed paraffin embedded human brain stained with MBP antibody .
Application
at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for detailed descript
Application
at a dilution of 1/1000, staining Myelin Basic Protein (MBP; Alexa 488 secondary at 1/2000) in the cortex and hippocampus of rat brain tissue (30m thick coronal sections). Staining was obtained following free floating IHC (see protocol link for d
Application
Detection of Myelin basic protein by IHC in Human Breast| Positive Control | Brain |
|---|---|
| Application Notes | For IHC-P: Use at a dilution of 1:50-1:100 in an ABC method. We suggest an incubation period of 30 minutes at RT. Optimal dilutions/concentrations should be determined by the researcher.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | Contains sodium azide |
