Application
IHC analysis of white matter in human brain tissue using Myelin basic protein antibody [22].| Product Name | Myelin basic protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [22] to Myelin basic proteinThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arra |
| Immunogen | Human myelin basic protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2 |
| Clone Number | 22 |
| Host Species | Mouse |
| Tested Applications | IHCIHC-FIHC-P |
| IHC:1:50~1:200 IHC-F:1:50~1:100 IHC-P:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | MBP myelin basic protein |
|---|---|
| Target | Myelin basic protein |
| Tissue Specificity | This antibody recognizes an epitope of myelin basic protein between amino acids 84-89. |
Application
IHC analysis of white matter in human brain tissue using Myelin basic protein antibody [22].| Application Notes | IHC:1:50~1:200 IHC-F:1:50~1:100 IHC-P:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Tissue Culture Supernatant with 0.09%Sodium Azide |
