Application
Myf5 antibody detects Myf5 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with Myf5 antibody diluted by 1:1000.| Product Name | Myf5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Myf5 (myogenic factor 5)Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human Myf5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rabbit, BovineMouse (100%), Rat (100%), Rabbit (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | bHLHc2 myogenic factor 5 MYF5 |
|---|---|
| Target | Myf5 |
| Molecular Weight(MW) | 28 kDa (note) |
| Cellular Localization | Nucleus |
Application
Myf5 antibody detects Myf5 protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12 % SDS-PAGE, and the membrane was blotted with Myf5 antibody diluted by 1:1000.| Positive Control | human muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
