Application
Madin Darby Canine Kidney (MDCK) was separated on SDS-PAGE and probed with AIA TO MYOSIN VI (KA-15) (RB) . The antibody was developed with Alk Phos APA Rabbit IgG (gt) and a NBT/BCIP substrate. Antibody dilution 1/2,000.WB
(A) Western blot analysis of MYO6 in the hind paw of normal and CIA mice. Data are means ± SD from four independent experiments. **P < 0.01 vs normal group.IHC
(B) Representative images of immunohistochemical staining with anti-MYO6 in the ankle of normal and CIA mice.IF
(A) Immunofluorescence of osteoclasts differentiated from WT and MYO6−/− BMM for MYO6, F-actin and nucleus with MYO6 antibody, rhodamine Phalloidin and DAPI respectively (original magnification, ×400). BMM were cultured in 24-well plates with glass cover slips under the stimulation of RANKL for 5 days, n = 6.| Product Name | MYO6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MYO6Myosins belong to a superfamily of actin based motor proteins comprising at least 15 or more classes. There are two main groups of myosins: the conventional (class II) and the unconventional myosins. Myosin VI is a relatively abundant widespread unconventional myosin composed of an N-terminal motor domain, a light chain binding neck region, a coil-coiled region, and a highly conserved C-terminal domain. At the converter region between the catalytic head and the neck region of myosin VI, there is a characteristic linker of approximately 50 amino acids. Native myosin VI is apparently a two-headed dimer of the heavy chains with each heavy chain bound to calmodulin light chain. Myosin VI is a unique reverse actin motor in vitro i.e. to display motility towards the pointed (minus) ends of actin filaments, a direction opposite to all other currently known myosins. |
| Immunogen | Synthetic peptide (Human) conjugated to KLH. |
| Conjugation | Agarose |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500~1:2000 ICC/IF:1:100~1:500 |
|
| Species Reactivity | DogHumanMousePigRat |
| Concentration | 1mg/ |
| Purification | Affinity purified |
| Gene Synonyms | MYR12 MYO5A Q9Y4I1 160777 MYO6 myosin Va MYH12 myosin V GS1 MYO5 MYOXIN 4644 |
|---|---|
| Target | MYO6 |
| Molecular Weight(MW) | 150 kDa |
| Cellular Localization | Cell Membrane, Cytoplasmic and Golgi Apparatus |
Application
Madin Darby Canine Kidney (MDCK) was separated on SDS-PAGE and probed with AIA TO MYOSIN VI (KA-15) (RB) . The antibody was developed with Alk Phos APA Rabbit IgG (gt) and a NBT/BCIP substrate. Antibody dilution 1/2,000.
WB
(A) Western blot analysis of MYO6 in the hind paw of normal and CIA mice. Data are means ± SD from four independent experiments. **P < 0.01 vs normal group.
IHC
(B) Representative images of immunohistochemical staining with anti-MYO6 in the ankle of normal and CIA mice.
IF
(A) Immunofluorescence of osteoclasts differentiated from WT and MYO6−/− BMM for MYO6, F-actin and nucleus with MYO6 antibody, rhodamine Phalloidin and DAPI respectively (original magnification, ×400). BMM were cultured in 24-well plates with glass cover slips under the stimulation of RANKL for 5 days, n = 6.| Positive Control | MDCK whole cell lysate (immunoblotting), NRK whole cell lysate (immunocytochemistry) |
|---|---|
| Application Notes | WB:1:500~1:2000 ICC/IF:1:100~1:500 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4oC short term. For extended storage aliquot and store at -20oC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15 mM Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
