Application
Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Myosin VI Clone: MUD-19 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. A431 2. MCF-7 3. MDA-MB-231| Product Name | MYO6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MUD - 19] to MYO6Myosins belong to a superfamily of actin based motor proteins comprising at least 15 or more classes. There are two main groups of myosins: the conventional (class II) and the unconventional myosins. Myosin VI is a relatively abundant widespread unconventional myosin composed of an N-terminal motor domain, a light chain binding neck region, a coil-coiled region, and a highly conserved C-terminal domain. At the converter region between the catalytic head and the neck region of myosin VI, there is a characteristic linker of approximately 50 amino acids. Native myosin VI is apparently a two-headed dimer of the heavy chains with each heavy chain bound to calmodulin light chain. Myosin VI is a unique reverse actin motor in vitro i.e. to display motility towards the pointed (minus) ends of actin filaments, a direction opposite to all other currently known myosins. |
| Immunogen | Synthetic peptide, corresponding to amino acids 291-302 of Human MYO6. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | MUD-19 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| ELISA: Use at an assay dependent concentration. WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 150 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | MYR12 MYO5A Q9Y4I1 160777 MYO6 myosin Va MYH12 myosin V GS1 MYO5 MYOXIN 4644 |
|---|---|
| Target | MYO6 |
| Cellular Localization | Cell Membrane, Cytoplasmic and Golgi Apparatus |
Application
Cell line lysates were separated on SDS-PAGE and probed with 0.5 μg/mL Monoclonal Anti-Myosin VI Clone: MUD-19 . The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. Lanes 1. A431 2. MCF-7 3. MDA-MB-231| Positive Control | A431 whole cell lysate |
|---|---|
| Application Notes | ELISA: Use at an assay dependent concentration. WB: Use at a concentration of 2 μg/ml. Detects a band of approximately 150 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 15% Sodium Azide; Constituents: 1% BSA, 0.01M PBS. pH 7.4 |
