Application
Whole cell extract of rat embryonic brain was separated on SDS-PAGE and blotted with Rabbit Anti-Myosin IX/Myr5 . The antibody was developed with Goat Anti-Rabbit IgG Peroxidase and chemiluminescent substrate. Lane A: Antibody dilution 1:1,000 Lane B: Antibody dilution 1: 1,000 + 20 mg/mL. Myosin IXb immunizing peptide.| Product Name | MYO9B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MYO9BMyosin IX belongs to the family of unconventional myosins, distinct from the classical myosins I and myosins II. Myosin IX, thought to be involved in signal transduction and leukocyte differentiation, includes several isoforms. The rat isoforms are myosin IX/Myr5 (230 kDa) and Myr7 (300 kDa), and the human isoforms are myosin IXa (MYO9A) and myosin IXb (MYO9B). The isoforms of myosin IX have similar overall domain structure and are expressed in many tissues and cell types, although they have distinct tissue expression patterns. The subcellular localization of class IX myosins appears to be partly cytoplasmic, and partly associated with membranes and the actin cytoskeleton. The expression level of myosins IX may vary during development and differentiation. |
| Immunogen | Synthetic peptide conjugated to KLH, corresponding to amino acids 1960-1980 of Rat MYO9B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. Detects a band of approximately 230 kDa using a rat embryonic brain extract. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Gene Synonyms | 25486 Myo9b |
|---|---|
| Target | MYO9B |
| Cellular Localization | Cytoplasmic |
Application
Whole cell extract of rat embryonic brain was separated on SDS-PAGE and blotted with Rabbit Anti-Myosin IX/Myr5 . The antibody was developed with Goat Anti-Rabbit IgG Peroxidase and chemiluminescent substrate. Lane A: Antibody dilution 1:1,000 Lane B: Antibody dilution 1: 1,000 + 20 mg/mL. Myosin IXb immunizing peptide.| Positive Control | Rat embryonic brain extract |
|---|---|
| Application Notes | WB: Use at a dilution of 1/1000. Detects a band of approximately 230 kDa using a rat embryonic brain extract. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 0.01M PBS. pH 7.4 |
