Application
Sample(30 µg of whole cell lysate) A:293T B:HeLa S3 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MyoD1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MyoD1 (myogenic differentiation 1)This gene encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite for myogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MyoD. The exact sequence is proprietary. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPWB |
| WB:1:500~1:2000 |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, BovineRat (93%), Pig (95%), Sheep (95%), Bovine (95%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MYF3 antibody MYOD antibody PUM antibody bHLHc1 antibody MYOD1 antibody myoblast determination protein 1 antibody myogenic factor 3 antibody myf-3 antibody class C basic helix-loop-helix protein 1 antibody myogenic differentiation 1 antibody |
|---|---|
| Target | MyoD |
| Molecular Weight(MW) | 35 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample(30 µg of whole cell lysate) A:293T B:HeLa S3 12% SDS PAGE diluted at 1:500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , A431 , H1299 , HeLaS3 , Raji , *CL1-0 , *CL1-5 |
|---|---|
| Application Notes | WB:1:500~1:2000 |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
