Application
Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: MYO1C transfected 293T lysate 5% SDS PAGE diluted at 1:5000Application
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using Myosin 1C antibody at 1:500 dilution.| Product Name | Myosin 1C antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Myosin 1C (myosin IC)This gene encodes a member of the unconventional myosin protein family, which are actin-based molecular motors. The protein is found in the cytoplasm, and one isoform with a unique N-terminus is also found in the nucleus. The nuclear isoform associates with RNA polymerase I and II and functions in transcription initiation. The mouse ortholog of this protein also functions in intracellular vesicle transport to the plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene. The related gene myosin IE has been referred to as myosin IC in the literature, but it is a distinct locus on chromosome 19. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Myosin 1C. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, BovineMouse (98%), Rat (98%), Xenopus laevis (80%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ23903 antibody MMI-beta antibody MMIb antibody NMI antibody myr2 antibody MYO1C antibody myosin I beta antibody nuclear myosin I antibody myosin-Ic antibody myosin-I beta antibody myosin IC antibody |
|---|---|
| Molecular Weight(MW) | 122 kDa (note) (3) |
| Cellular Localization | Cytoplasm , Cell membrane (By similarity) , Cell projection , stereocilium membrane (By similarity) , Isoform 3: Nucleus , nucleoplasm , Nucleus , nucleolus , nuclear pore complex |
Application
Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: MYO1C transfected 293T lysate 5% SDS PAGE diluted at 1:5000
Application
Immunohistochemical analysis of paraffin-embedded PC14 xenograft, using Myosin 1C antibody at 1:500 dilution.| Positive Control | A431 , MYO1C transfected 293T lysate |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
