Rabbit polyclonal to Skeletal and Smooth MyosinMyosin is a 500 kD protein known to interact with actin in muscle and in non-muscle cells. It contains two identical heavy chains (200 kD each) and four light chains (15-20 kD each). Myosin molecules consist of two major regions; the tail (rod) and head. These molecules aggregate into filaments through the tail region and interact with actin and ATP through the head region.
Immunogen
Full length native protein (purified) (Cow).
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
ICC/IF
IF: Use at a dilution of 1/10. Optimal dilutions/concentrations should be determined by the end user.:
Species Reactivity
HumanMouseBovine
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Target
Myosin skeletal + smooth
Tissue Specificity
Immunofluorescent staining of cultured human and mouse fibroblasts shows a striated pattern of labeling along stress fibers. The antibody specifically stains the A-band of skeletal muscle.
Cellular Localization
Plasma membrane
Positive Control
Human and Mouse fibroblast cell line slides
Application Notes
IF: Use at a dilution of 1/10. Optimal dilutions/concentrations should be determined by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.