Application
Western blot analysis of extracts from MCF-7/Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | N4BP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to N4BP1Inhibitor of the E3 ubiquitin-protein ligase ITCH. Acts by interacting with the second WW domain of ITCH, leading to compete with ITCH's substrates and impairing ubiquitination of substrates. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human N4BP1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NEDD4binding protein1 NEDD4-binding protein 1 N4BP1 NEDD4binding protein 1 NEDD4 binding protein 1 |
|---|---|
| Target | N4BP1 |
| Molecular Weight(MW) | 100 kDa (note) |
| Tissue Specificity | N4BP1 Antibody detects endogenous levels of total N4BP1 protein. |
| Cellular Localization | Primarily localizes to the nucleolus. Also localizes to the PML nuclear bodies, when desumoylated. |
Application
Western blot analysis of extracts from MCF-7/Jurkat/293 cells, using N4BP1 Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
