Application
Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 μg/mL.Application
Western blot analysis of N4BP1 in HeLa cell lysate with N4BP1 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.Application
Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 μg/mL.| Product Name | N4BP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | N4BP1 Antibody: Nedd4-binding partner-1 (N4BP1) is a developmentally expressed protein interactor and monoubiquitylation substrate of Nedd4, a HECT domain-containing ubiquitin ligase that mediates ubiquitylation and proteasome degradation of target proteins and may have a fundamental role to play in embryonic processes. N4BP1 can undergo Nedd4-mediated polyubiquitylation and proteosomal degradation. N4BP1 also functions as a negative regulator of AIF4 (also known as ITCH or Atrophin-1), an E3-ubiquitin ligase, by interacting with the second WW domain of AIF4 and impairing ubiquitination of substrates.1) Murillas R, Simms KS, Hatakeyama S, et al. Identification of developmentally expressed proteins that functionally interact with Nedd4 ubiquitin ligase. J. Biol. Chem. 2002; 277:2897-907. |
| Immunogen | N4BP1 antibody was raised against a 18 amino acid synthetic peptide near the amino terminus of human N4BP1. The immunogen is located within the first 50 amino acids of N4BP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | N4BP1 |
|---|---|
| Alternative Names | N4BP1 Antibody: KIAA0615 NEDD4-binding protein 1 N4BP1 |
| Sequence Similarities | 2) Cat. No. 17-001 - HeLa Cell Slide |
| Tissue Specificity | At least three isoforms of N4BP1 are known to exist; this antibody will only detect the longest isoform. |
Application
Immunocytochemistry of N4BP1 in HeLa cells with N4BP1 antibody at 10 μg/mL.
Application
Western blot analysis of N4BP1 in HeLa cell lysate with N4BP1 antibody at 0.5 μg/mL in (A) the absence and (B) the presence of blocking peptide.
Application
Immunofluorescence of N4BP1 in HeLa cells with N4BP1 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
|---|---|
| Application Notes | N4BP1 antibody can be used for detection of N4BP1 by Western blot at 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL. |
| Form | Liquid |
|---|---|
| Storage Instructions | N4BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | N4BP1 Antibody is supplied in PBS containing 0.02% sodium azide. |
