Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 10% SDS PAGE diluted at 1:1000Application
NAB1 antibody detects NAB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAB1 protein stained by NAB1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | NAB1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NAB1 (NGFI-A binding protein 1 (EGR1 binding protein 1))Acts as a transcriptional repressor for zinc finger transcription factors EGR1 and EGR2. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NAB1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (86%), Rat (85%), Rhesus Monkey (99%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | NAB1 antibody NGFI-A-binding protein 1 antibody EGR-1-binding protein 1 antibody transcriptional regulatory protein p54 antibody EGR1 binding protein 1 antibody NGFI-A binding protein 1 (EGR1 binding protein 1) antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (5) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: HeLa nucleus 10% SDS PAGE diluted at 1:1000
Application
NAB1 antibody detects NAB1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAB1 protein stained by NAB1 antibody diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | HeLa nucleus |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
