Application
Immunoprecipitation of NAB2 protein from Jurkat whole cell extracts using 5 μg of NAB2 antibody . Western blot analysis was performed using NAB2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NAB2 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:2000Application
NAB2 antibody detects NAB2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NAB2 antibody dilution: 1:500.| Product Name | NAB2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NAB2 (NGFI-A binding protein 2 (EGR1 binding protein 2))This gene encodes a member of the family of NGFI-A binding (NAB) proteins, which function in the nucleus to repress transcription induced by some members of the EGR (early growth response) family of transactivators. NAB proteins can homo- or hetero-multimerize with other EGR or NAB proteins through a conserved N-terminal domain, and repress transcription through two partially redundant C-terminal domains. Transcriptional repression by the encoded protein is mediated in part by interactions with the nucleosome remodeling and deactylase (NuRD) complex. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NAB2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (98%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MADER antibody MGC75085 antibody NAB2 antibody EGR1 binding protein 2 antibody melanoma-associated delayed early response protein antibody EGR-1-binding protein 2 antibody NGFI-A-binding protein 2 antibody NGFI-A binding protein 2 (EGR1 binding prot |
|---|---|
| Molecular Weight(MW) | 57 kDa (note) (2) |
| Cellular Localization | Nucleus (By similarity) |
Application
Immunoprecipitation of NAB2 protein from Jurkat whole cell extracts using 5 μg of NAB2 antibody . Western blot analysis was performed using NAB2 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using NAB2 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:2000
Application
NAB2 antibody detects NAB2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. NAB2 antibody dilution: 1:500.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
