Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using NAGLU antibody at 1:500 dilution.Application
NAGLU antibody [N3C1], Internal detects NAGLU protein at cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody [N3C1], Internal diluted at 1:200. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.Application
NAGLU antibody [N3C1], Internal detects NAGLU protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NAGLU antibody [N3C1], Internal diluted at 1:500.Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.| Product Name | NAGLU antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NAGLU (N-acetylglucosaminidase, alpha)This gene encodes an enzyme that degrades heparan sulfate by hydrolysis of terminal N-acetyl-D-glucosamine residues in N-acetyl-alpha-D-glucosaminides. Defects in this gene are the cause of mucopolysaccharidosis type IIIB (MPS-IIIB), also known as Sanfilippo syndrome B. This disease is characterized by the lysosomal accumulation and urinary excretion of heparan sulfate. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NAGLU. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | BovineBovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MPS-IIIB antibody MPS3B antibody NAG antibody UFHSD antibody NAGLU antibody alpha-N-acetylglucosaminidase antibody N-acetyl-alpha-glucosaminidase antibody N-acetylglucosaminidase alpha antibody |
|---|---|
| Molecular Weight(MW) | 82 kDa (note) (2) |
Application
Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.
Application
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma, using NAGLU antibody at 1:500 dilution.
Application
NAGLU antibody [N3C1], Internal detects NAGLU protein at cytoplasm by immunofluorescent analysis. Sample: U-87 MG cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NAGLU protein stained by NAGLU antibody [N3C1], Internal diluted at 1:200. Red: beta Tubulin 3/ TUJ1 protein stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:200. Blue: Hoechst 33342 staining.
Application
NAGLU antibody [N3C1], Internal detects NAGLU protein at cytoplasm in mouse brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. NAGLU antibody [N3C1], Internal diluted at 1:500.
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.
Application
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with NAGLU antibody [N3C1], Internal diluted at 1:1000.| Positive Control | U87-MG , SK-N-SH , IMR32 , SK-N-AS , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
