Application
Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.Application
Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.| Product Name | NAK antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [108A429] to NAKNuclear factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-kB activity. Phosphorylation of I-kB by IkB kinase (IKK) complex leads to degradation of I-kB and activation of NF-kB. The IKK complex contains IKKa,IKKb, and IKKg. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kB-activating kinase), and T2K. NAK/TBK1 activates IKKb through direct phosphorylation. NAK/TBK1is activated by growth factors and PMA and mediates IKK and NF-kB activation in response to growth factors. NAK/TBK1 functions upstream of NIK and the IKK complex. NAK/TBK1 is also critical in protecting embryonic liver from apoptosis. |
| Immunogen | Full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 108A429 |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 87.5 kDa). For optimal results, primary antibody incubations should be performed at room temperature.ICC/IF,IHC:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 29110 604834 T2K TBK1 Q9UHD2 FLJ11330 NAK |
|---|---|
| Target | NAK |
| Tissue Specificity | recognises human NAK. |
| Cellular Localization | Cytoplasmic |
Application
Lysate from 293 cells untransfected (lane 1) and transfected with human TBK1 cDNA (lane 2) probed with TBK1 antibody at 2 ug/ml.
Application
Human Daudi (A) and mouse RAW (B) cell lysate probed with TBK1 antibody at 2 ug/ml.| Positive Control | 293 cell extracts transfected with human TBK1 cDNA |
|---|---|
| Application Notes | WB: Use at a concentration of 1 - 2 μg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 87.5 kDa). For optimal results, primary antibody incubations should be performed at room temperature.ICC/IF,IHC:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.02% Sodium Azide; Constituents: PBS |
