Application
IHC analysis of rat hypothalamus using NALCN antibody (green) and DAPI (blue). (C) Merged image of A and B. SON: supra-optic nucleus; OT: optic tract.Application
WB analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and SH-SYS5 (lanes 3 and 6) lysates using NALCN antibody at a dilution of 1:200 in the absence (lens 1-3) or presence (lens 4-6) of blocking peptide.Application
ICC/IF analysis of PC12 cells using NALCN antibody at a dilution of 1:50. Merged image of extracellular staining with live image of the cells.| Product Name | NALCN antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NALCNmay act as a voltage activated cation channel [RGD, Feb 2006] |
| Immunogen | Peptide (C)SMMFESPFRRVMH, corresponding to amino acid residues 902-914 of rat Sodium leak channel non-selective protein (Accession Q6Q760). Extracellular, S1-S2 domain III. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nalcn Vgcnl1 sodium leak channel non-selective |
|---|---|
| Target | NALCN |
Application
IHC analysis of rat hypothalamus using NALCN antibody (green) and DAPI (blue). (C) Merged image of A and B. SON: supra-optic nucleus; OT: optic tract.
Application
WB analysis of rat brain (lanes 1 and 4), mouse heart (lanes 2 and 5) and SH-SYS5 (lanes 3 and 6) lysates using NALCN antibody at a dilution of 1:200 in the absence (lens 1-3) or presence (lens 4-6) of blocking peptide.
Application
ICC/IF analysis of PC12 cells using NALCN antibody at a dilution of 1:50. Merged image of extracellular staining with live image of the cells.| Application Notes | ICC/IF:1:200~1:500 IHC:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline (PBS), pH 7.4, 1% BSA and 0.05% NaN3. |
