Application
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Detection of Sodium Potassium ATPase Alpha 1 in fixed Hela cells.Application
Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Immunocytochemistry: Detection of ATPA1 (Green) in HepG2 cells using . Nuclei (Blue) were counterstained using Hoechst 33258.Application
Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Staining of enodmetrial glands within the uterus using . Note the absence of staining in the surrounding myometrial smooth muscle.Application
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.Application
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Detection of Sodium Potassium ATPase Alpha 1 in human postmortem prefrontal cortex tissue.Application
Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) Staining of enodmetrial glands within the uterus using Note the absence of staining in the surrounding myometrial smooth muscle.Application
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.Application
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Detection of Sodium Potassium ATPase Alpha 1 in fixed Hela cells.| Product Name | NaK ATPase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to NaK-ATPaseNa,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and beta subunit. The alpha-polypeptide has been shown to be the catalytically active subunit, wherease the Beta-polypeptide appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase alpha-1 subunit, in brain, is expressed in both neuronal and non-neuronal cells. |
| Immunogen | Purified Na,K-ATPase from rabbit renal outer medulla. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 464.6 or 6H |
| Host Species | Mouse |
| Tested Applications | FACSICC/IFIHCWB |
| For WB: Use at a diltuion of 1:1000-1:10000.Western blot analysis gives a distinct band at 112 kDa. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celcius appears to work fine). For FACS:1:50-1:200.For ICC/IF:1:50-1:1000.For IHC:1:200.Optimal dilutions and concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseDogDrosophilaPigRatYeastGuinea PigPrimateRabbitSheepXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 29110 604834 T2K TBK1 NaK ATPase NAK Q9UHD2 FLJ11330 |
|---|---|
| Target | NaK ATPase |
| Tissue Specificity | This antibody is specific for Na,K-ATPase alpha 1 subunit |
| Light Chain Type | kappa |
Application
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Detection of Sodium Potassium ATPase Alpha 1 in fixed Hela cells.
Application
Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Immunocytochemistry: Detection of ATPA1 (Green) in HepG2 cells using . Nuclei (Blue) were counterstained using Hoechst 33258.
Application
Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Staining of enodmetrial glands within the uterus using . Note the absence of staining in the surrounding myometrial smooth muscle.
Application
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.
Application
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Detection of Sodium Potassium ATPase Alpha 1 in human postmortem prefrontal cortex tissue.
Application
Immunohistochemistry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) Staining of enodmetrial glands within the uterus using Note the absence of staining in the surrounding myometrial smooth muscle.
Application
Western Blot: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Analysis detecting Na, K-ATPase (alpha) in porcine proximal tubule protein.
Application
Flow Cytometry: Sodium Potassium ATPase Alpha 1 Antibody (464.6) - Detection of Sodium Potassium ATPase Alpha 1 in fixed Hela cells.| Application Notes | For WB: Use at a diltuion of 1:1000-1:10000.Western blot analysis gives a distinct band at 112 kDa. Do not boil the sample prior to loading on the gel for Western Blot (60 degrees Celcius appears to work fine). For FACS:1:50-1:200.For ICC/IF:1:50-1:1000.For IHC:1:200.Optimal dilutions and concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.1% sodium azide |
