Application
Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).Application
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using . Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.Application
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates usingApplication
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using| Product Name | NaK ATPase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal to human NaK - ATPaseNa,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and Beta subunit. The a-polypeptide has been shown to be the catalytically active subunit, whereas the Beta-polypeptide is glycosylated and appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. |
| Immunogen | Purified Na,K-ATPase from rabbit renal outer medulla. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | 464.8 or 8A |
| Host Species | Mouse |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000. ICC/IF:1:500-1:1000.: |
|
| Species Reactivity | HumanDogPigRatHamsterRabbitXenopusBovine |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 29110 604834 T2K TBK1 NaK ATPase NAK Q9UHD2 FLJ11330 |
|---|---|
| Target | NaK ATPase |
| Tissue Specificity | This antibody is specific for Na,K-ATPase beta 1 subunit |
| Light Chain Type | kappa |
Application
Immunocytochemistry/Immunofluorescence: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) - Sodium Potassium ATPase Beta 1 antibody was tested in HepG2 cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Application
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) - Detection of Na, K-ATPase (beta) in rat kidney homogenates (20 ug)using . Lane 1: 1:2,500 primary. Lane 2: 1:5,000 primary.
Application
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) - Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using
Application
Western Blot: Sodium Potassium ATPase Beta 1 Antibody (464.8 (also known as 8A)) Western blot analysis of Sodium Potassium ATPase Beta 1 expression in 1) SK-BR-3 and 2) A-431 whole cell lysates using| Application Notes | WB:1:1000-1:5000. ICC/IF:1:500-1:1000.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
