Application
Western blot analysis of Nanos2 in human testis lyate with Nanos2 antibody at (A) 2 and (B) 4 ug/ml.| Product Name | Nanos2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Nanos2Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos2 antibody will not cross-react with either Nanos 1 or Nanos3. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nanos2 Nanos homolog 2 Nanos homolog2 |
|---|---|
| Target | Nanos2 |
| Tissue Specificity | This antibody is specific for Nanos2 C-Terminus |
Application
Western blot analysis of Nanos2 in human testis lyate with Nanos2 antibody at (A) 2 and (B) 4 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85392-PEP - Nanos2 Peptide Lysates: Cat. No. GTX15374 - Human Testis Tissue Lysate |
|---|---|
| Application Notes | WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
