Application
Western blot analysis of Nanos3 in human brain tissue lysate with Nanos3 antibody at 2 ug/ml.Application
Immunohistochemistry of Nanos3 in human brain tissue with Nanos3 antibody at 2.5 ug/ml.Application
Immunofluorescence of Nanos3 in Human Brain cells with Nanos3 antibody at 20 ug/mL.| Product Name | Nanos3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to Nanos3Nanos is a zinc-finger containing, RNA-binding protein that has been implicated in germ cell development in both invertebrates and vertebrates. In drosophila, Nanos represses apoptosis during development to ensure proper germ-line development. Unlike Nanos1 whose expression in mice is dispensable, the Nanos2 and Nanos3 proteins are required for germ cell development. Nanos2-null primordial germ cells (PGCs) die only in the male gonads and show no defects in females, while Nanos3-null PGCs are lost during the migration stage regardless of sex. Nanos2 and Nanos3 have distinct expression patterns during embryo development, suggesting that these two proteins do not have redundant functions. However, expression of Nanos2 can at least partially replace Nanos3 function in a Nanos3-null background. Nanos3 expression can not rescue Nanos2-null defects. This Nanos3 antibody will not cross-react with either Nanos 1 or Nanos2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Nanos homolog3 Nanos homolog 3 Nanos3 |
|---|---|
| Target | Nanos3 |
| Tissue Specificity | This antibody is specific for Nanos3 C-Terminus |
Application
Western blot analysis of Nanos3 in human brain tissue lysate with Nanos3 antibody at 2 ug/ml.
Application
Immunohistochemistry of Nanos3 in human brain tissue with Nanos3 antibody at 2.5 ug/ml.
Application
Immunofluorescence of Nanos3 in Human Brain cells with Nanos3 antibody at 20 ug/mL.| Positive Control | Blocking Peptide: |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 IHC-P:1:50~1:200 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
