Rabbit Polyclonal antibody to NARFL (nuclear prelamin A recognition factor-like)Component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for maturation of extramitochondrial Fe/S proteins. Seems to negatively regulate the level of HIF1A expression, although this effect could be indirect.
Immunogen
Recombinant protein encompassing a sequence within the center region of human NARFL. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Rhesus Monkey, BovineMouse (89%), Rat (88%), Rhesus Monkey (98%), Bovine (87%)
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
FLJ21988 antibody HPRN antibody IOP1 antibody LET1L antibody PRN antibody NARFL antibody protein related to Narf antibody iron-only hydrogenase-like protein 1 antibody cytosolic Fe-S cluster assembly factor NARFL antibody LET1 like/JFP15 antibody
Molecular Weight(MW)
53 kDa (note) (3)
Cellular Localization
Nucleus
Application
Application
Sample (30 ug of whole cell lysate) A: MCF-7 10% SDS PAGE diluted at 1:3000
Positive Control
MCF-7
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.