Application
Staining of human peripheral blood lymphocytes with Mouse anti Human NCAMApplication
Staining of human peripheral blood lymphocytes with Mouse anti Human NCAM:BiotinApplication
Surface staining of human peripheral blood cells with anti-CD56 (MEM-188): FITC .| Product Name | NCAM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [MEM - 188] to NCAMNCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. |
| Immunogen | Tissue / cell preparation (Human). (KG-1 acute myelogenous leukaemia cell line). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | MEM-188 |
| Host Species | Mouse |
| Tested Applications | FACSIPWB |
| FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CD56 116930 NCAM MSK39 P13591 4684 NCAM1 |
|---|---|
| Target | NCAM |
| Tissue Specificity | CD56 antigen (human). This antibody reacts with 180 kDa isoform characteristic for leucocytes. Reactivity with other NCAM isoforms has not been tested. |
| Cellular Localization | Type I membrane protein |
Application
Staining of human peripheral blood lymphocytes with Mouse anti Human NCAM
Application
Staining of human peripheral blood lymphocytes with Mouse anti Human NCAM:Biotin
Application
Surface staining of human peripheral blood cells with anti-CD56 (MEM-188): FITC .| Application Notes | FACS: Use at an assay dependant concentration. IP: Use at an assay dependant concentration. WB: Use at an assay dependant concentration. Use under non-reducing conditions. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline pH 7.4 containing 15mM sodium azide |
