| Product Name | NCAM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal to NCAMThis antibody reacts with a 135/220 kD glycosylated and sialyated protein, expressed on CD2+, CD3-, CD16+ natural killer cells, and neuroectodermal cells.This antibody positively recognizes certain areas within the brain, notably the cerebellum, hypothalamus, hippocampus, substantia nigra and spinal cord. |
| Immunogen | TE-71 cells (Medulloblastoma Cell Line) |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | T199 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-F |
| Immunohistochemistry (Frozen), Flow Cytometry. For a three-step avidin-biotin complex system, a dilution of 1:25 to 1:50 may be used as a guideline. The optimal conditions should be determined by the individual laboratory. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | CD56 116930 NCAM MSK39 P13591 4684 NCAM1 |
|---|---|
| Target | NCAM |
| Cellular Localization | Cell Membrane |
| Application Notes | Immunohistochemistry (Frozen), Flow Cytometry. For a three-step avidin-biotin complex system, a dilution of 1:25 to 1:50 may be used as a guideline. The optimal conditions should be determined by the individual laboratory. Dilute according to the particular application being used. In general, the 0.05M Borate pH 8.0 containing 0.15M Sodium Chloride, 0.05% Sodium Azide, is a good diluent to use with most antibodies.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, and 0.05% Sodium Azide, pH 7.5. |
