| Product Name | NCAM antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [UJ13A] to NCAM (CD56)NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig)-like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma. |
| Immunogen | 16 week human foetal brain |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | UJ13A |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 120 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | CD56 116930 NCAM MSK39 P13591 4684 NCAM1 |
|---|---|
| Target | NCAM |
| Cellular Localization | Type I membrane protein |
| Application Notes | IF: Use at an assay dependent dilution. WB: Use at an assay dependent dilution. Predicted molecular weight: 120 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C. Long term (>6months) aliquot and store at -20°C. Avoid repeated freeze thawing. |
| Storage Buffer | Phosphate buffered saline |
