Application
Western Blot analysis of UBE2F expression in transfected 293T cell line by UBE2F polyclonal antibody. A: Non-transfected lysate. B: UBE2F transfected lysate. 12% SDS PAGE diluted at 1:500| Product Name | NCE2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to NCE2 (UBE2F) (ubiquitin-conjugating enzyme E2F (putative))Accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX2, but not RBX1, suggests that the RBX2-UBE2F complex neddylates specific target proteins, such as CUL5. |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human NCE2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (100%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC18120 antibody NCE2 antibody UBE2F antibody ubiquitin-conjugating enzyme E2 F antibody NEDD8-conjugating enzyme 2 antibody NEDD8 carrier protein UBE2F antibody NEDD8-conjugating enzyme UBE2F antibody NEDD8 protein ligase UBE2F antibody ubiquitin |
|---|---|
| Target | NCE2 (UBE2F) |
| Molecular Weight(MW) | 21 kDa (note) (5) |
Application
Western Blot analysis of UBE2F expression in transfected 293T cell line by UBE2F polyclonal antibody. A: Non-transfected lysate. B: UBE2F transfected lysate. 12% SDS PAGE diluted at 1:500| Positive Control | NCE2 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
