Application
Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the antigen-specific peptide.Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | NCF1 (phospho Ser345) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCF1 (phospho Ser345)The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008] |
| Immunogen | Peptide sequence around phosphorylation site of Serine 345(P-Q-S(p)-P-G) derived from Human Nuclear p47 phox. |
| Modification | p-Ser345 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| WB: 1:500 - 1:1000. IHC: 1:50 - 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SH3PXD1A NCF1A neutrophil cytosolic factor 1 p47phox NCF1 NOXO2 |
|---|---|
| Target | NCF1 (phospho Ser345) |
| Tissue Specificity | The antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345. |
Application
Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the antigen-specific peptide.
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | WB: 1:500 - 1:1000. IHC: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C/1 year |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
