WB
Western blot analysis of p47-phox on different lysates with Mouse anti-p47-phox antibody at 1/2,000 dilution.Lysates/proteins at 30 µg/Lane.Exposure time: 43 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | p47-phox Recombinant Mouse Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human p47-phox aa 51-250. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | WB |
| WB:1:2000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NCF1 |
|---|---|
| Gene Synonyms | CGD1 NCF-1 NCF1A NOXO2 NCF-47K p47phox SH3PXD1A p47-phox |
| Gene Full Name | neutrophil cytosolic factor 1 |
| Gene Summary | The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008] |
| Alternative Names | 47 kDa autosomal chronic granulomatous disease protein antibody 47 kDa neutrophil oxidase factor antibody NADPH oxidase organizer 2 antibody NCF 47K antibody NCF-1 antibody NCF-47K antibody Ncf1 antibody NCF1_HUMAN antibody Neutrophil cytosol factor 1 antibody Neutrophil cytosolic factor 1 antibody 47 kDa autosomal chronic granulomatous disease protein antibody 47 kDa neutrophil oxidase factor antibody NADPH oxidase organizer 2 antibody NCF 47K antibody NCF-1 antibody NCF-47K antibody Ncf1 antibody NCF1_HUMAN antibody Neutrophil cytosol factor 1 antibody Neutrophil cytosolic factor 1 antibody neutrophil cytosolic factor 1 (chronic granulomatous disease autosomal 1) antibody Neutrophil NADPH oxidase factor 1 antibody Nox organizer 2 antibody Nox organizing protein 2 antibody Nox-organizing protein 2 antibody NOXO2 antibody p47 phox antibody p47-phox antibody SH3 and PX domain containing protein 1A antibody SH3 and PX domain-containing protein 1A antibody SH3PXD1A antibody |
| Molecular Weight(MW) | 45 kDa |
| Cellular Localization | Cytoplasm, cytosol, Membrane. |
WB
Western blot analysis of p47-phox on different lysates with Mouse anti-p47-phox antibody at 1/2,000 dilution.Lysates/proteins at 30 µg/Lane.Exposure time: 43 seconds;4-20% SDS-PAGE gel.Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.. |
