Western blot analysis of extracts from Hela cells using p47 phox (Phospho-Ser359) Antibody. The lane on the left is treated with the antigen-specific peptide.
Rabbit Polyclonal antibody to NCF1 (phospho Ser359)The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]
Immunogen
Peptide sequence around phosphorylation site of Serine 359 (Q-R-S(p)-K-P) derived from Human p47 phox.
Modification
p-Ser359
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
WB: 1:500 - 1:1000:
Species Reactivity
Human
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
The antibody detects endogenous level of p47 phox only when phosphorylated at Serine 359.
Application
Application
Western blot analysis of extracts from Hela cells using p47 phox (Phospho-Ser359) Antibody. The lane on the left is treated with the antigen-specific peptide.
Application Notes
WB: 1:500 - 1:1000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20˚C/1 year
Storage Buffer
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.