Application
NCF1 antibody detects NCF1 protein at cytoplasm by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NCF1 protein stained by NCF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCF1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:5000| Product Name | NCF1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NCF1 (neutrophil cytosolic factor 1)The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human NCF1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rabbit, BovineMouse (83%), Rat (80%), Pig (82%), Rabbit (86%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ79451 antibody NCF1A antibody NOXO2 antibody SH3PXD1A antibody p47phox antibody NCF1 antibody NCF-47K antibody SH3 and PX domain-containing protein 1A antibody 47 kDa autosomal chronic granulomatous disease protein antibody NCF-1 antibody nox |
|---|---|
| Molecular Weight(MW) | 45 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
NCF1 antibody detects NCF1 protein at cytoplasm by immunofluorescent analysis. Sample: Raji cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NCF1 protein stained by NCF1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunohistochemical analysis of paraffin-embedded Hela xenograft, using NCF1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Raji 10% SDS PAGE diluted at 1:5000| Positive Control | Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
